Journal article
DEFICIENT REPAIR OF CISPLATIN-DNA ADDUCTS IDENTIFIED IN HUMAN TESTICULAR TERATOMA CELL-LINES ESTABLISHED FROM TUMORS FROM UNTREATED PATIENTS
BT HILL, KJ SCANLON, J HANSSON, A HARSTRICK, M PERA, AMJ FICHTINGERSCHEPMAN, SA SHELLARD
EUROPEAN JOURNAL OF CANCER | PERGAMON-ELSEVIER SCIENCE LTD | Published : 1994
Abstract
Germ cell tumour lines appear generally more sensitive in vitro to cisplatin than other cultured cell lines, reflecting their clinical responsiveness. We proposed (Cancer Res 1988, 48, 3019-3024) that cisplatin hypersensitivity, expressed by a testicular teratoma line (SuSa), might be explained by an inability to repair platinated DNA. We have now quantitated cisplatin cytotoxicity by clonogenic assay, and platinum (Pt)-DNA adduct formation and removal immunochemically in four other testicular teratoma continuous cell lines (GCT46, GCT27 clone 4, H32 and H12.1), all established from tissue from non-drug-treated patients. For 1-h in vitro drug exposures, the cisplatin concentration required t..
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