Journal article

Expression of CD30 and CD30 ligand in cultured cell lines from human germ-cell tumors

MF Pera, W Bennett, DP Cerretti

LABORATORY INVESTIGATION | WILLIAMS & WILKINS | Published : 1997

Abstract

Embryonal carcinoma (EC) cells are the multipotent stem cells of human teratocarcinomas. EC cell lines isolated from human testicular teratocarcinomas retain pluripotentiality and show cell-surface marker phenotype and growth characteristics similar to those of primate embryonic stem cells. CD30, a member of the tumor necrosis factor superfamily of cytokine receptors, is expressed on the surface of human EC cells, but little is known regarding its function or the expression of its ligand in this context. Northern blot analysis, reverse transcriptase-PCR, and immunoprecipitation were used to study the expression of CD30 ligand and CD30 in cell lines representing multipotent and nullipotent EC..

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University of Melbourne Researchers