Conference Proceedings

Mechanism of the hypertension produced by inhibition of nitric oxide biosynthesis in rats

M Zambetis, GJ Dusting, S Rajanayagam, OL Woodman

Journal of Cardiovascular Pharmacology | Published : 1991


We have examined the effects of inhibition of nitric oxide (NO) biosynthesis on the blood pressure (BP) of anesthetized rats using N-nitro-L-arginine (NOLA). Infusion of NOLA (5, 15, or 60 mg/kg) caused a rise in BP of up to 155 mm Hg that could be prevented (but not reversed) by administration of L-arginine (300 and 600 mg/kg, i.v.). In addition, NOLA (5-60 mg/kg) reduced the duration of the depressor response to acetylcholine (1 μg/kg), but not that of sodium nitroprusside (10 μg/kg). In rats treated with NOLA (15 mg/kg), infusion of the K+ channel antagonist glibenclamide (30 mg/kg) failed to inhibit the residual depressor response to acetylcholine, indicating that this response is not du..

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