Journal article
A rapid method for obtaining mesenchymal stem cells and platelets from bone marrow aspirate
B Dozza, G Gobbi, E Lucarelli, M Pierini, CD Bella, T Frisoni, PL Tazzari, F Ricci, P Mirandola, C Carubbi, S Giannini, D Donati, M Vitale
Journal of Tissue Engineering and Regenerative Medicine | WILEY | Published : 2014
DOI: 10.1002/term.1551
Open access
Abstract
Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) are currently used alone or in combination for therapeutic applications especially for bone repair. We tested whether MSCs can be isolated from bone marrow (BM) aspirate using a commercially available kit commonly used to obtain PRP from peripheral blood (PB). Results revealed that mononuclear cells and platelets from both PB and BM could be efficiently isolated by obtaining a mononuclear and platelet rich fraction (PB-MPRF and BM-MPRF, respectively). Starting with comparable volumes, the number of platelets increased 1.5-fold in BM-MPRF compared to PB-MPRF. The number of clonogenic cells in BM-MPRF samples was significantly higher..
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Funding Acknowledgements
The authors are grateful to Ms. Elena Brandano, Ms. Sharon Perrone and Ms. Elizabeth Olive for reading the manuscript and Ms. Cristina Ghinelli for editorial assistance. In addition, we would like to thank Dr. Sean O'Connell and Dr. Roberto Beretta for helpful comments. This work was supported by Grant "Regenerative medicine in osteo-articular diseases" carried out in the framework of the collaboration Emilia Romagna: Region-University 2007-2009 Research Programme.