αB crystallin translocation and phosphorylation: Signal transduction pathways and preconditioning in the isolated rat heart

Abstract

In this program of studies we have characterized in detail the translocation (assessed by Triton-insolubility) and phosphorylation (using serine-45 or -59 phosphospecific antibodies) of αB crystallin during myocardial ischemia [both with or without ischemic preconditioning (IPC)]. Pharmacological activators and inhibitors allowed us to characterize the signaling pathways involved in αB crystallin phosphorylation during ischemia. Ischemic preconditioning alone caused 30% of the heart's αB crystallin pool to translocate, providing a significant translocation 'head-start' in protected tissue. This enhanced translocation is coupled with increased (3-fold) αB crystallin phosphorylation at both se..