Journal article

What assay is optimal for the diagnosis of measles virus infection? An evaluation of the performance of a measles virus real-time reverse transcriptase PCR using the Cepheid SmartCycler(®) and antigen detection by immunofluorescence.

Kyra YL Chua, Kiran Thapa, Chaturangi M Yapa, Lucy K Somerville, Sharon C-A Chen, Dominic E Dwyer, Vicky Sheppeard, Jen Kok

Journal of Clinical Virology | Published : 2015

Abstract

BACKGROUND: Despite the World Health Organization (WHO)-reported elimination of measles in Australia, importation of cases especially in travellers from Asia continues in Sydney, Australia's largest city. Laboratory confirmation supports clinico-epidemiological evidence of measles virus infection, and is needed to establish elimination. OBJECTIVES: To evaluate the performance of a random access real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay using the moderate complexity SmartCycler(®) platform, and measles antigen detection by immunofluorescence (IFA), for the detection of measles virus in patient samples. STUDY DESIGN: One hundred samples comprising nose and throat..

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University of Melbourne Researchers