Journal article

MATURATION AND DIFFERENTIATION OF CULTURED FETAL STOMACH - EFFECTS OF CORTICOSTEROIDS, PENTAGASTRIN, AND CYTOCHALASIN-B

ND YEOMANS, JS TRIER, PC MOXEY, ET MARKEZIN

GASTROENTEROLOGY | W B SAUNDERS CO | Published : 1976

Abstract

Gastric mucosa of fetal rats undergoes striking developmental changes during the last few days of gestation in utero. To investigate some aspects of this process, gastric explants from 18-day fetuses (4 days before birth) were maintained in organ culture for 3 days, then assessed by light and electron microscopy. The epithelia from base line uncultured stomachs were stratified and morphologically undifferentiated. During culture in basic medium (Leibovitz L 15), modest maturation of antral and fundic architecture occurred, characterized by epithelial invagination to produce small pit-glands. Secretory granules appeared in occasional epithelial cells, and cytochemistry indicated that most wer..

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University of Melbourne Researchers