Journal article

REGULATION OF ALKALINE-PHOSPHATASE EXPRESSION IN A NEONATAL RAT CLONAL CALVARIAL CELL STRAIN BY RETINOIC ACID

KW NG, PR GUMMER, VP MICHELANGELI, JF BATEMAN, T MASCARA, WG COLE, TJ MARTIN

JOURNAL OF BONE AND MINERAL RESEARCH | WILEY | Published : 1988

Abstract

A clonal cell strain, UMR 201, was established from a culture of rat calvarial cells by the process of limiting dilution on a collagen substratum. One-day-old neonatal rat calvaria stripped of periosteum were placed on collagen in alpha-MEM with 10% fetal bovine serum (FBS). Cells that grew out from the calvaria were passaged eight times to select cells with the ability to proliferate in culture before cloning was attempted. Cells from the clonal strain were homogeneous in appearance with a doubling time in culture of about 24 hours. The UMR 201 cells formed predominantly type 1 collagen. When treated with retinoic acid (RA), all cells showed an intense staining for alkaline phosphatase (ALP..

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