Journal article

DNA METHYLATION AND GENETIC INACTIVATION AT THYMIDINE KINASE LOCUS - 2 DIFFERENT MECHANISMS FOR SILENCING AUTOSOMAL GENES

A DOBROVIC, JLP GAREAU, G OUELLETTE, WEC BRADLEY

SOMATIC CELL AND MOLECULAR GENETICS | SPRINGER/PLENUM PUBLISHERS | Published : 1988

Abstract

Patterns of methylation of CpG dinucleotides in the promoter region of the thymidine kinase (TK) gene in wild-type and TK-deficient Chinese hamster cell lines were studied. Whereas wild-type cells were unmethylated, three conventionally derived TK-deficient cell lines were all almost completely methylated in the promoter region. Demethylation at a number of different CpG sites was observed upon selection for reexpression of the TK gene. Of thirteen HhaI (GCGC) or HpaII (CCGG) sites studied, the highest correlation between absence of methylation and at least partial TK activity was obtained at one HhaI site within 20 bp of the putative cap site. Silencing in the three conventionally derived m..

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