Journal article

Somatic mutations, acetylator status, and prognosis in colorectal cancer

JE Hardingham, WJ Butler, D Roder, A Dobrovic, RB Dymock, RE Sage, IC Roberts-Thomson

GUT | BRITISH MED JOURNAL PUBL GROUP | Published : 1998

Abstract

BACKGROUND: Somatic mutations in K-ras and TP53 may be associated with both acetylator status and prognosis in colorectal cancer. AIMS: To determine whether cancers with somatic mutations are more frequent in fast acetylators and whether mutations or acetylator status influence prognosis after colorectal surgery. PATIENTS: One hundred consecutive subjects undergoing elective surgery for colorectal cancer. METHODS: Acetylator status was determined by polymerase chain reaction (PCR) genotyping for polymorphism in the N-acetyltransferase 2 (NAT2) gene. Mutations in K-ras (codon 12) and TP53 were determined by PCR analysis using restriction enzyme digestion and single strand conformation polymor..

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University of Melbourne Researchers