Characterization of two populations of CFU‐S fractionated from mouse fetal liver by fluorescence‐activated cell sorting

Abstract

The properties of spleen colony forming units (CFU‐S) contained in two murine fetal liver cell populations (pre‐colony‐forming cell (CFC) and CFC fractions) obtained after fluorescence‐activated cell sorting fractionation on the basis of pokeweed mitogen binding have been analyzed. Individual spleen colonies generated by both populations were examined to determine cellularity, differential cell morphology, as well as CFU‐S and in vitro CFC content. Spleen colonies from both populations contained approximately equal numbers of cells 7 days after transplantation; at 12 days, however, CFU‐S from the pre‐CFC fraction produced four times as many cells per colony than colonies from the CFC fractio..