Affinity Conversion of Receptors for Colony Stimulating Factors: Properties of Solubilized Receptors
Nicos A Nicola, Dale Cary
Growth Factors | TAYLOR & FRANCIS LTD | Published : 1992
Murine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) and Multi-CSF (interleukin-3) can exist in both high- and low-affinity forms and demonstrate trans-modulation by several different ligands. In contrast the recently cloned human GM-CSF receptor and murine interleukin-3 (IL-3) receptor display only low-affinity binding. To begin to understand the molecular basis of the formation of high- and low-affinity receptors and their trans-modulation we have developed methods for the solubilization and assay of GM-CSF and interleukin-3 receptors so that their binding characteristics can be studied in cell-free solution. Both receptors displayed a single class of high-affinit..View full abstract
Awarded by National Institutes of Health, Bethesda
This work was supported by the National Health and Medical Research Council, Canberra, Australia; the National Institutes of Health, Bethesda, Grant CA22556; the Anti-Cancer Council of Victoria, the J. D. and L. Hams Trust Fund; the Philip Bushell Trust; and AMRAD Corporation, Australia. D. Metcalf is thanked for fruitful discussions.