Journal article

TRANSFORMATION OF SINGLE MYELOID PRECURSOR CELLS BY THE MALIGNANT HISTIOCYTOSIS SARCOMA-VIRUS (MHSV) - GENERATION OF GROWTH-FACTOR-INDEPENDENT MYELOID COLONIES AND PERMANENT CELL-LINES

K KLINGLER, GR JOHNSON, NA NICOLA, G ARMAN, N KLUGE, W OSTERTAG

JOURNAL OF CELLULAR PHYSIOLOGY | WILEY-LISS | Published : 1988

Abstract

Direct single-cell assays for oncogenic transformation are available for fibroblasts but not for other cell types. Using malignant histiocytosis sarcoma virus (MHSV), a member of the ras family of retroviruses, in vivo-infected granulocyte/macrophage and macrophage precursor cells lost the requirement for externally added hematopoietic growth factors. Factor-independent growth was demonstrated by colony-transfer experiments. More than 25% of the independent colonies were established as permanent macrophage cell lines following a phase of adaptation to tissue culture conditions. Factor-independent colony growth was also obtained by in vitro infection of single cells. As many as 50% of all mye..

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