Functional analysis of mature hematopoietic cells from mice lacking the βc chain of the granulocyte-macrophage colony-stimulating factor receptor

Abstract

Mice with a null mutation of the βc chain of the granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and IL-5 receptors (βc-null mice) develop an alveolar proteinosis-like lung disease. The pathogenesis of this disease is uncertain and, although a defect in alveolar macrophage function has been postulated, no previous analysis of mature hematopoietic cells in mice with alveolar proteinosis has been reported. Therefore, we undertook a functional analysis of the mature hematopoietic cell compartment in βc-null mice. In addition, we reexamined the roles of the GM-CSF receptor α chain and the βc chain in signaling by GM-CSF. Neutrophils and macrophages from βc-null m..