Purification of hemopoietic progenitor cells from human marrow using a fucose-binding lectin and cell sorting

Abstract

Human peripheral blood granulocytes, but not lymphocytes, erythrocytes, or monocytes, bound the fucose-binding lectin from Lotus tetragonolobus (FBP), and this binding was competitively inhibited by the sugar α-L-fucose. The fluorescence-activated cell sorter was used to study the appearance of this receptor on human marrow cells during granulocyte differentiation and to prepare fractions enriched for granulocyte-macrophage progenitor cells (granulocyte-macrophage colony-forming cells - GM-CFC). Cell binding of fluoresceinated FBP increased for bone marrow cells in the sequence - lymphocytes, blast cells, promyelocytes and myelocytes, monocytes, and polymorphonuclear cells. Selection of cell..