Journal article

CHARACTERISTICS OF SOLUBLE AND MEMBRANE-BOUND FORMS OF HEMATOPOIETIC GROWTH-FACTOR RECEPTORS - RELATIONSHIPS TO BIOLOGICAL FUNCTION

NA NICOLA

CIBA FOUNDATION SYMPOSIA | JOHN WILEY & SONS LTD | Published : 1990

Abstract

In order to understand how extracellular growth factors (colony-stimulating factors, CSFs) induce biological responses in haemopoietic cells it is necessary first to describe the primary binding characteristics of isolated receptors and then to determine how living cells modify these binding characteristics and process the ligand-receptor complexes. We have solubilized multi-CSF (interleukin 3) and granulocyte macrophage CSF (GM-CSF) receptors in 1% Triton X-100 and developed methods to study their binding characteristics in solution. Whilst the multi-CSF receptor exhibits the same binding characteristics in solution and in the cellular state, the GM-CSF receptor appears to be converted to a..

View full abstract

University of Melbourne Researchers