Conference Proceedings

Rat model of chronic tympanic membrane perforation: A longitudinal histological evaluation of underlying mechanisms.

Allen Y Wang, Lawrence J Liew, Yi Shen, Jeffrey T Wang, Magnus von Unge, Marcus D Atlas, Rodney J Dilley

Int J Pediatr Otorhinolaryngol | Published : 2017

Abstract

OBJECTIVE: To evaluate histologically the progressive development and underlying mechanisms of chronic tympanic membrane perforation (TMP) in a rat model using a two-weeks ventilation tube (VT) treatment combined with topical application of mitomycin C/dexamethasone (VT-M/D), compared with normal tympanic membrane and acute TMPs. METHODS: Fifty male Sprague-Dawley rats were divided into three experimental groups: a normal control group (n = 5), an acute TMP group (n = 5) (i.e. 3 days post-myringotomy) and a VT-M/D group (n = 40). The TMs were regularly assessed by otoscopy. The normal control animals were sacrificed on day 0 and the acute TMP group was sacrificed 3 days post-myringotomy for ..

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