Capacity to form choroid plexus-like cells in vitro is restricted to specific regions of the mouse neural ectoderm

Abstract

Neural ectoderm was dissected from 9.5-day and 8.5-day gestation mouse embryos and divided into forebrain, midbrain, hindbrain and spinal cord regions. Forebrain and hindbrain material from 9.5-day neural ectoderm was further divided into presumptive choroid plexus regions and regions that would normally form nervous tissue in vivo. All tissues were plated onto a basement membrane substratum for culture in vitro. It was found that explants of neural ectoderm that would normally form choroid plexus in vivo, readily differentiated to form choroid plexus-like cells in culture. Cells from hindbrain segments and forebrain regions, which would normally form nervous tissue, also had the potential t..