Journal article

Derivation of an endogenous small RNA from double-stranded Sox4 sense and natural antisense transcripts in the mouse brain

King-Hwa Ling, Peter J Brautigan, Sarah Moore, Rachel Fraser, Pike-See Cheah, Joy M Raison, Milena Babic, Young Kyung Lee, Tasman Daish, Deidre M Mattiske, Jeffrey R Mann, David L Adelson, Paul Q Thomas, Christopher N Hahn, Hamish S Scott

GENOMICS | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2016

Abstract

Natural antisense transcripts (NATs) are involved in cellular development and regulatory processes. Multiple NATs at the Sox4 gene locus are spatiotemporally regulated throughout murine cerebral corticogenesis. In the study, we evaluated the potential functional role of Sox4 NATs at Sox4 gene locus. We demonstrated Sox4 sense and NATs formed dsRNA aggregates in the cytoplasm of brain cells. Over expression of Sox4 NATs in NIH/3T3 cells generally did not alter the level of Sox4 mRNA expression or protein translation. Upregulation of a Sox4 NAT known as Sox4ot1 led to the production of a novel small RNA, Sox4_sir3. Its biogenesis is Dicer1-dependent and has characteristics resemble piRNA. Expr..

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Grants

Awarded by National Health and Medical Research Council


Awarded by Malaysian Ministry of Science, Technology & Innovation (MOSTI) Science Fund


Awarded by Malaysian Ministry of Higher Education Fundamental Research Grant Scheme


Funding Acknowledgements

This work was supported by National Health and Medical Research Council fellowships [171601 and 461204 to H.S.S.], National Health and Medical Research Council Grants [219176, 257501 and 257529 to H.S.S.], Malaysian Ministry of Science, Technology & Innovation (MOSTI) Science Fund (02-01-04-SF2336 to K.H.L.), Malaysian Ministry of Higher Education Fundamental Research Grant Scheme (04-01-15-1663FR to P.S.C.), and a fellowship from Pfizer Australia [to P.Q.T.]. We would like to express our gratitude to Professor Greg Goodall from SA Pathology at IMVS, South Australia for his constructive comments and views regarding the manuscript.