Journal article

Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response

M Kocan, S Schaffer, T Ishige, U Sorger-Herrmann, VF Wendisch, M Bott

Journal of Bacteriology | AMER SOC MICROBIOLOGY | Published : 2006


Corynebacterium glutamicum contains genes for 13 two-component signal transduction systems. In order to test for their essentiality and involvement in the adaptive response to phosphate (Pi) starvation, a set of 12 deletion mutants was constructed. One of the mutants was specifically impaired in its ability to grow under Pi limitation, and therefore the genes lacking in this strain were named phoS (encoding the sensor kinase) and phoR (encoding the response regulator). DNA microarray analyses with the C. glutamicum wild type and the DeltaphoRS mutant supported a role for the PhoRS system in the adaptation to Pi starvation. In contrast to the wild type, the DeltaphoRS mutant did not induce th..

View full abstract