Journal article

Detection of GPCR/beta-Arrestin Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer Technology

Martina Kocan, Kevin DG Pfleger, WR Leifert

Methods in molecular biology (Clifton, N.J.) | HUMANA PRESS INC | Published : 2009


Bioluminescence resonance energy transfer (BRET) is a powerful and increasingly popular technique for studying protein-protein interactions in live cells and real time. In particular, there has been considerable interest in the ability to monitor interactions between G protein-coupled receptors (GPCRs) and proteins that serve as key regulators of receptor function, such as beta-arrestin. The BRET methodology involves heterologous co-expression of genetically fused proteins that link one protein of interest (e.g., a GPCR) to a bioluminescent donor enzyme and a second protein of interest (e.g., beta-arrestin) to an acceptor fluorophore. If the fusion proteins are in close proximity, resonance ..

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University of Melbourne Researchers