Journal article

Study of GPCR-Protein Interactions by BRET

Martina Kocan, Kevin DG Pfleger, GB Willars, RAJ Challiss

Methods in Molecular Biology | HUMANA PRESS INC | Published : 2011

Abstract

Bioluminescence resonance energy transfer (BRET) has become an extremely valuable technology for the real-time study of protein-protein interactions in live cells. This technique is highly amenable to the monitoring of G protein-coupled receptor (GPCR)-protein interactions, especially involving scaffolding, regulatory and signaling proteins, such as β-arrestins, which are now known to have significant roles in addition to receptor desensitization. The BRET procedure utilizes heterologous coexpression of fusion proteins linking one protein of interest (e.g. a GPCR) to a bioluminescent donor enzyme, a variant of Renilla luciferase, and a second protein of interest (e.g. β-arrestin) to an accep..

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University of Melbourne Researchers