Profoundly Reduced CD1c( ) Myeloid Dendritic Cell HLA-DR and CD86 Expression and Increased Tumor Necrosis Factor Production in Experimental Human Blood-Stage Malaria Infection
Jessica R Loughland, Gabriela Minigo, Julie Burel, Peta E Tipping, Kim A Piera, Fiona H Amante, Christian R Engwerda, Michael F Good, Denise L Doolan, Nicholas M Anstey, James S McCarthy, Tonia Woodberry
INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2016
Dendritic cells (DCs) are sentinels of the immune system that uniquely prime naive cells and initiate adaptive immune responses. CD1c (BDCA-1) myeloid DCs (CD1c(+) mDCs) highly express HLA-DR, have a broad Toll-like receptor (TLR) repertoire, and secrete immune modulatory cytokines. To better understand immune responses to malaria, CD1c(+) mDC maturation and cytokine production were examined in healthy volunteers before and after experimental intravenous Plasmodium falciparum infection with 150- or 1,800-parasite-infected red blood cells (pRBCs). After either dose, CD1c(+) mDCs significantly reduced HLA-DR expression in prepatent infections. Circulating CD1c(+) mDCs did not upregulate HLA-DR..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
This study was supported by the Australian National Health and Medical Research Council (NHMRC) (project grant 1021198, program grant 1037304, and fellowships to N.M.A., J.S.M., C.E., D.L.D., M.F.G., and G.M.). The views expressed in this publication are those of the authors and do not reflect the views of the NHMRC. J.R.L. was supported in part by an APA Ph.D. scholarship; J.B. was supported in part by a University of Queensland International Scholarship.