Style proteins of a wild tomato (Lycopersicon peruvianum) associated with expression of self-incompatibility.
SL Mau, EG Williams, A Atkinson, MA Anderson, EC Cornish, B Grego, RJ Simpson, A Kheyr-Pour, AE Clarke
Planta | Published : 1986
The identification, isolation and aminoterminal sequencing of two S-genotype-associated proteins from style extracts of Lycopersicon peruvianum Mill. is reported. There is a high level of homology between these two sequences and with the amino-terminal sequences of other S-allele-associated glycoproteins isolated from Nicotiana alata Link et Otto. These sequences were obtained by a new high-sensitivity method of selected twodimensional gel analysis followed by electroelution and purification of proteins by inverse-gradient high-performance liquid chromatography before sequencing.