Quantification and enzyme targets of fatty acid amides from duckweed root exudates involved in the stimulation of denitrification

Abstract

Fatty acid amides from plant root exudates, such as oleamide and erucamide, have the ability to participate in strong plant-microbe interactions, stimulating nitrogen metabolism in rhizospheric bacteria. However, mechanisms of secretion of such fatty acid amides, and the nature of their stimulatory activities on microbial metabolism, have not been examined. In the present study, collection, pre-treatment, and determination methods of oleamide and erucamide in duckweed root exudates are compared. The detection limits of oleamide and erucamide by gas chromatography (GC) (10.3 ng mL-1 and 16.1 ng mL-1, respectively) are shown to be much lower than those by liquid chromatography (LC) (1.7 and 5...