GSA-1/ARG1 protects root gravitropism in Arabidopsis under ammonium stress
Na Zou, Baohai Li, Hao Chen, Yanhua Su, Herbert J Kronzucker, Liming Xiong, Frantisek Baluska, Weiming Shi
NEW PHYTOLOGIST | WILEY | Published : 2013
Gravitropism plays a critical role in plant growth and development, plant stability and acclimation to changes in water and nutrient availability. Ammonium (NH4(+)) is well known to have profound effects on root growth, but its impacts on gravitropism are poorly understood. To determine which genes are essential for the maintenance of root gravitropism under NH4(+) stress, we isolated and identified an NH4 (+)-sensitive mutant, gsa-1 (gravitropism sensitive to ammonium-1), in Arabidopsis thaliana, using an agar plate root reorientation assay. We found that, under NH4(+) stress, gsa-1 displayed increased loss of root gravitropism. Gene cloning and sequencing revealed that gsa-1 contains a G t..View full abstract
Awarded by National Natural Science Foundation of China
Awarded by Chinese Academy Sciences Innovation Program
Awarded by Natural Sciences and Engineering Research Council of Canada (NSERC)
We thank Professors Malcolm Bennett (University of Nottingham), Ben Scheres (Utrecht University) and Tom Guilfoyle (University of Missouri) for providing the transgenic lines of Arabidopsis, and the Arabidopsis Biological Resource Center for the mutant seeds. We are grateful to Dr Jianru Zuo (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for kind provision of Arabidopsis seeds mutagenized with T-DNA transformation, Feifei Sun (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for assistance with amyloplast staining and Dr Guangjie Li (Institute of Soil Science, Chinese Academy of Sciences) for assistance with DR5::GUS staining. We also thank other members of our team and members of the laboratory of Professor Yanhua Su (Institute of Soil Science, Chinese Academy of Sciences) for their assistance. This work was supported by the National Natural Science Foundation of China (31200189 and 30771285), the Chinese Academy Sciences Innovation Program (ISSASIP1103) and the Natural Sciences and Engineering Research Council of Canada (NSERC, Discovery Grant 217277-2009).