Journal article

Ammonium-induced shoot ethylene production is associated with the inhibition of lateral root formation in Arabidopsis

Guangjie Li, Baohai Li, Gangqiang Dong, Xiaoyu Feng, Herbert J Kronzucker, Weiming Shi

JOURNAL OF EXPERIMENTAL BOTANY | OXFORD UNIV PRESS | Published : 2013

Abstract

Foliar NH4(+) exposure is linked to inhibition of lateral root (LR) formation. Here, the role of shoot ethylene in NH4(+)-induced inhibition of LR formation in Arabidopsis was investigated using wild-type and mutant lines that show either blocked ethylene signalling (etr1) or enhanced ethylene synthesis (eto1, xbat32). NH4(+) exposure of wild-type Arabidopsis led to pronounced inhibition of LR production chiefly in the distal root, and triggered ethylene evolution and enhanced activity of the ethylene reporter EBS:GUS in the shoot. It is shown that shoot contact with NH4(+) is necessary to stimulate shoot ethylene evolution. The ethylene antagonists Ag(+) and aminoethoxyvinylglycine (AVG) mi..

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University of Melbourne Researchers

Grants

Awarded by National Basic Research Program of China


Awarded by National Natural Science Foundation of China


Awarded by Chinese Academy Sciences Innovation Program


Awarded by Natural Sciences and Engineering Research Council of Canada (NSERC)


Funding Acknowledgements

We thank Tom J. Guilfoyle (University of Missouri) for providing DR5:GUS transgenic Arabidopsis seeds, and Dr J. Alonso at North Carolina State University for providing seeds of the EBS:GUS reporter lines which were generated by Dr Anna Stepanova in Dr Joe Ecker's lab, and the Arabidopsis Biological Resource Centre of Ohio State University for mutant seeds. We also thank Dr Weifeng Xu (Institute of Soil Science, Chinese Academy of Sciences) for assistance with Arabidopsis seeds. This work was supported by the National Basic Research Program of China (2007CB109303), the National Natural Science Foundation of China (30771285, 41171234, and 31200189), the Chinese Academy Sciences Innovation Program (grant no. ISSASIP1103), and the Natural Sciences and Engineering Research Council of Canada (NSERC, Discovery Grant 217277-2009).