Journal article

COMPARTMENTATION AND FLUX CHARACTERISTICS OF NITRATE IN SPRUCE

HJ KRONZUCKER, MY SIDDIQI, ADM GLASS

PLANTA | SPRINGER | Published : 1995

Abstract

The radiotracer 13N was used to undertake compartmental analyses for NO(Formula presented.)in intact non-mycorrhizal roots of Picea glauca (Moench) Voss. seedlings. Three compartments were defined, with half-lives of exchange of 2.5 s, 20 s, and 7 min. These were identified as representing surface adsorption, apparent free space, and cytoplasm, respectively. Influx, efflux, and net flux as well as cytoplasmic and apparent-free-space nitrate concentrations were estimated for three different concentration regimes of external nitrate. After exposure to external NO(Formula presented.)for 3 d, influx was calculated to be 0.09 μmol·g−1·h−1 (at 10 μM [NO(Formula presented.)]o), 0.5μmol·g−1·h−1 (at ..

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