In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
Massilva Rahmoun, Rowena Lavery, Sabine Laurent-Chaballier, Nicolas Bellora, Gayle K Philip, Moira Rossitto, Aleisha Symon, Eric Pailhoux, Florence Cammas, Jessica Chung, Stefan Bagheri-Fam, Mark Murphy, Vivian Bardwell, David Zarkower, Brigitte Boizet-Bonhoure, Philippe Clair, Vincent R Harley, Francis Poulat
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2017
In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell S..View full abstract
Awarded by National Health and Medical Research Council Program
Awarded by National Health and Medical Research Council Fellowship
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
Agence Nationale pour la Recherche (ANR blanc Testis-Dev to B.B. and F.P.); National Health and Medical Research Council Program [1074258 to V.R.H.]; National Health and Medical Research Council Fellowship [1020034 to V.R.H.]; Victorian Government's Operational Infrastructure Support Program (to V.R.H.); Australian Postgraduate Award (to A.S.). Funding for open access charge: Agence Nationale pour la Recherche.