Journal article
Molecular and immunological characterisation of tropomyosin from Anisakis pegreffii
A Asnoussi, IE Aibinu, RB Gasser, AL Lopata, PM Smooker
Parasitology Research | SPRINGER | Published : 2017
Abstract
Tropomyosin (TM) is a major allergen in shellfish, known to cross-react with mite, cockroach and/or some roundworm (nematode) TM. In this study, we aimed to express and purify TM from the parasitic nematode Anisakis pegreffii and also to characterise its cross-reactivity with TM from shellfish. A. pegreffii was isolated from the flathead tiger fish (Neoplatycephalus richardsoni) and characterised using single-strand conformation polymorphism (SSCP)-based sequencing of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA. The recombinant tropomyosin (rTM) of A. pegreffii was expressed, purified and confirmed by immunohistochemistry, sequencing and LC-MS..
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Funding Acknowledgements
Andreas Lopata received financial support via an ARC Future Fellowship for this work. Robin Gasser received support from the Australian Research Council (ARC), the National Health and Medical Research Council (NHMRC) of Australia, Yourgene Bioscience (Taiwan), Melbourne Water Corporation and The University of Melbourne.