Journal article
The superoxide dismutase mimetic tempol blunts diabetes-induced upregulation of NADPH oxidase and endoplasmic reticulum stress in a rat model of diabetic nephropathy
MJ De Blasio, A Ramalingam, AH Cao, D Prakoso, JM Ye, R Pickering, AMD Watson, JB de Haan, DM Kaye, RH Ritchie
European Journal of Pharmacology | ELSEVIER SCIENCE BV | Published : 2017
Abstract
Endoplasmic reticulum (ER) stress contributes to progression of diabetic nephropathy, which promotes end-stage renal failure in diabetic patients. This study was undertaken to investigate the actions of tempol and ramipril, pharmacological agents that target the consequences of NADPH oxidase, on diabetic nephropathy in a rat model of type 1 diabetes, with an emphasis on markers of ER stress. Male Sprague-Dawley rats were injected intravenously with a single bolus of streptozotocin (55 mg/kg) to induce type 1 diabetes. An additional age-matched group of rats was administered with citrate vehicle as controls. After 4 weeks of untreated diabetes, rats received tempol (1.5 mM/kg/day subcutaneous..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported in part by a grant-in-aid from Diabetes Australia and by the Victorian Government of Australia's Operational Infrastructure Support Program. RHR (ID1059960) is supported by Senior Research Fellowship from the National Health and Medical Research Council (NHMRC) of Australia. AMDW is an honorary member of the Baker Heart & Diabetes Institute. AR was supported by an Endeavour Research Fellowship from the Department of Education Australia. We acknowledge the Monash Micro-Imaging (MMI) facility for provision of instrumentation and training.