Cannula-based drug delivery to the guinea pig round window causes a lasting hearing loss that may be temporarily mitigated by BDNF
Phillip JP Sale, Aaron Uschakov, Tasfia Saief, David P Rowe, Carla J Abbott, Chi D Luu, Amy J Hampson, Stephen J O'Leary, David J Sly
HEARING RESEARCH | ELSEVIER SCIENCE BV | Published : 2017
Sustained local delivery of drugs to the inner ear may be required for future regenerative and protective strategies. The round window is surgically accessible and a promising delivery route. To be viable, a delivery system should not cause hearing loss. This study determined the effect on hearing of placing a drug-delivery microcatheter on to the round window, and delivering either artificial perilymph (AP) or brain-derived neurotrophic factor (BDNF) via this catheter with a mini-osmotic pump. Auditory brainstem responses (ABRs) were monitored for 4 months after surgery, while the AP or BDNF was administered for the first month. The presence of the microcatheter - whether dry or when delive..View full abstract
The authors would like to thank Michelle Stirling (animal husbandry), Scott Chambers and Leon Winata (technical assistance), Prue Nielsen (histological processing) and James Fallon (Igor software routine development) for their assistance. This work was funded by the Garnett Passe and Rodney Williams Memorial Foundation, Melbourne, Australia.