Describing the role of Drosophila melanogaster ABC transporters in insecticide biology using CRISPR-Cas9 knockouts
Shane Denecke, Roberto Fusetto, Philip Batterham
Insect Biochemistry and Molecular Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2017
ABC transporters have a well-established role in drug resistance, effluxing xenobiotics from cells and tissues within the organism. More recently, research has been dedicated to understanding the role insect ABC transporters play in insecticide toxicity, but progress in understanding the contribution of specific transporters has been hampered by the lack of functional genetic tools. Here, we report knockouts of three Drosophila melanogaster ABC transporter genes, Mdr49, Mdr50, and Mdr65, that are homologous to the well-studied mammalian ABCB1 (P-glycoprotein). Each knockout mutant was created in the same wild type background and tested against a panel of insecticides representing different c..View full abstract
The authors would like to thank the University of Melbourne for the Writing Up Scholarship and the Melbourne International Research Scholarship granted to S.D. and R.F. that supported the research reported here. Furthermore, we acknowledge Nate Ferraro for assisting in editing the manuscript. Lastly, we must thank Michael Murray of the University of Melbourne for providing fly facilities during the renovations of our own flyroom.