Localized synchrotron irradiation of mouse skin induces persistent systemic genotoxic and immune responses

J Ventura; PN Lobachevsky; JS Palazzolo; H Forrester; NM Haynes; A Ivashkevich; AW Stevenson; CJ Hall; A Ntargaras; V Kotsaris; GC Pollakis; G Potsi; K Skordylis; G Terzoudi; IS Pateras; VG Gorgoulis; AG Georgakilas; CN Sprung; OA Martin

Journal article

Localized synchrotron irradiation of mouse skin induces persistent systemic genotoxic and immune responses

J Ventura, PN Lobachevsky, JS Palazzolo, H Forrester, NM Haynes, A Ivashkevich, AW Stevenson, CJ Hall, A Ntargaras, V Kotsaris, GC Pollakis, G Potsi, K Skordylis, G Terzoudi, IS Pateras, VG Gorgoulis, AG Georgakilas, CN Sprung, OA Martin

Cancer Research | AMER ASSOC CANCER RESEARCH | Published : 2017

DOI: 10.1158/0008-5472.CAN-17-1066

Abstract

The importance of nontargeted (systemic) effects of ionizing radiation is attracting increasing attention. Exploiting synchrotron radiation generated by the Imaging and Medical Beamline at the Australian Synchrotron, we studied radiation-induced nontargeted effects in C57BL/6 mice. Mice were locally irradiated with a synchrotron X-ray broad beam and a multiplanar microbeam radiotherapy beam. To assess the influence of the beam configurations and variations in peak dose and irradiated area in the response of normal tissues outside the irradiated field at 1 and 4 days after irradiation, we monitored oxidatively induced clustered DNA lesions (OCDL), DNA double-strand breaks (DSB), apoptosis, an..

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University of Melbourne Researchers

Nicole Haynes Author

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

All authors were supported by the Australian National Health and Medical Research Council (NHMRC) grant #10275598. H. Forrester and C.N. Sprung were supported by the 2010 round of the priority-driven Collaborative Cancer Research Scheme (grant #1002743), by the Australian Government Department of Health and Ageing with the assistance of Cancer Australia. A.W. Stevenson and C.J. Hall were supported by the Australian Synchrotron for use of the IMBL and facilities. J. Ventura was supported by the Felix Meyer Scholarship in Obstetrics and Gynecology at the Faculty of Medicine, Dentistry and Healthy Sciences, the University of Melbourne. A.G. Georgakilas was supported by the EU (grant #MC-CIG-303514), COST Action CM1201 "Biomimetic Radical Chemistry," Research Projects for Excellence IKY/SIEMENS, and the DAAD (grant #57339330).