Journal article
Selenium metabolism in cancer cells: The combined application of XAS and XFM techniques to the problem of selenium speciation in biological systems
CM Weekley, JB Aitken, L Finney, S Vogt, PK Witting, HH Harris
Nutrients | MDPI | Published : 2013
DOI: 10.3390/nu5051734
Abstract
Determining the speciation of selenium in vivo is crucial to understanding the biological activity of this essential element, which is a popular dietary supplement due to its anti-cancer properties. Hyphenated techniques that combine separation and detection methods are traditionally and effectively used in selenium speciation analysis, but require extensive sample preparation that may affect speciation. Synchrotron-based X-ray absorption and fluorescence techniques offer an alternative approach to selenium speciation analysis that requires minimal sample preparation. We present a brief summary of some key HPLC-ICP-MS and ESI-MS/MS studies of the speciation of selenium in cells and rat tissu..
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Awarded by U.S. DOE
Awarded by Australian Research Council
Funding Acknowledgements
A549 cells were a gift from Aviva Levina (The University of Sydney). We thank Ian Musgrave (The University of Adelaide) for assistance with cell culture. Use of the Advanced Photon Source at Argonne National Laboratory, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science by Argonne National Laboratory, was supported by the U.S. DOE under Contract No. DE-AC02-06CH11357. We gratefully acknowledge funding provided by the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron and funded by the Australian Government, the Australian Research Council (DP0985807 to HHH) and the Australian Synchrotron Postgraduate Award (CMW).