Journal article
Distinct cellular fates for KP1019 and NAMI-A determined by X-ray fluorescence imaging of single cells
JB Aitken, S Antony, CM Weekley, B Lai, L Spiccia, HH Harris
Metallomics | ROYAL SOC CHEMISTRY | Published : 2012
DOI: 10.1039/c2mt20072d
Abstract
Small molecule ruthenium complexes show great promise as anticancer pharmaceuticals, but further rational development of these as drugs is stymied by an incomplete understanding of the mechanisms that give rise to markedly different biological behaviour for structurally similar species. X-ray fluorescence imaging at two incident energies was used to reveal the intracellular distribution of Ru in single human cells treated with KP1019, showing Ru localised in both cytosol and in the nuclear region. In addition the imaging showed that treatment with KP1019 modulated Fe distribution to resemble the Ru distribution, without affecting cellular Fe content. In stark contrast, Ru could not be visual..
View full abstractGrants
Awarded by Australian Research Council
Awarded by USA Dept of Energy, Office of Science
Funding Acknowledgements
The authors thank David Benjafield for his generous assistance in figure preparation. This work was funded by the Australian Research Council (DP0985807-QEII to H.H.H, DP0984722 to H.H.H.) We acknowledge travel funding provided by the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron and funded by the Australian Government. The use of the Advanced Photon Source was supported by the USA Dept of Energy, Office of Science, under contract no. W-31-109-Eng-38.