Journal article
Uptake, distribution, and speciation of selenoamino acids by human cancer cells: X-ray absorption and fluorescence methods
CM Weekley, JB Aitken, S Vogt, LA Finney, DJ Paterson, MD De Jonge, DL Howard, IF Musgrave, HH Harris
Biochemistry | AMER CHEMICAL SOC | Published : 2011
DOI: 10.1021/bi101678a
Abstract
Selenium compounds exhibit chemopreventative properties at supranutritional doses, but the efficacy of selenium supplementation in cancer prevention is dependent on the chemical speciation of the selenium supplement and its metabolites. The uptake, speciation, and distribution of the common selenoamino acid supplements, selenomethionine (SeMet) and Se-methylselenocysteine (MeSeCys), in A549 human lung cancer cells were investigated using X-ray absorption and fluorescence spectroscopies. X-ray absorption spectroscopy of bulk cell pellets treated with the selenoamino acids for 24 h showed that while selenium was found exclusively in carbon-bound forms in SeMet-treated cells, a diselenide compo..
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Awarded by Australian Research Council
Awarded by U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences
Funding Acknowledgements
[ "This research was supported under the Australian Research Council's Discovery Projects funding scheme (DP0984722 and DP0985807, QEII). We acknowledge travel funding provided by the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron. The ISAP is an initiative of the Australian Government being conducted as part of the National Collaborative Research Infrastructure Strategy.", "A549 cells were a gift from Aviva Levina (The University of Sydney). Graham N. George (University of Saskatchewan) provided Se K-edge X-ray absorption spectra of model Se compounds. Use of the Advanced Photon Source at Argonne National Laboratory was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract DE-AC02-06CH11357. Parts of this research were conducted at the Stanford Synchrotron Radiation Labnoratory, a national user facility operated by Stanford University on behalf of the U.S. Department of Energy, Office of Basic Energy Sciences, and at the X-ray Fluorescence Microprobe beamline at the Australian Synchrotron." ]