Journal article
Characterization of the Human Pancreas Side Population as a Potential Reservoir of Adult Stem Cells
P Augstein, T Loudovaris, E Bandala-Sanchez, P Heinke, G Naselli, L Lee, WJ Hawthorne, LJ Góñez, AM Neale, F Vaillant, HE Thomas, TW Kay, I Banakh, LC Harrison
Pancreas | LIPPINCOTT WILLIAMS & WILKINS | Published : 2018
Abstract
Objectives The side population (SP) contains cells with stem cell/progenitor properties. Previously, we observed that the mouse pancreas SP expanded after pancreatic injury. We aimed to characterize the SP in human pancreas as a potential source of stem cells. Methods Human organ donor pancreata were fractionated into islets and exocrine tissue, enriched by tissue culture and dispersed into single cells. Cells were phenotyped by flow cytometry, and the SP was defined by efflux of fluorescent dye Hoechst 33342 visualized by ultraviolet excitation. Cells were flow sorted, and their colony-forming potential measured on feeder cells in culture. Results An SP was identified in islet and exocrine ..
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Awarded by Juvenile Diabetes Research Foundation International
Funding Acknowledgements
This study was supported by a Special Program Grant (465199) from the Australian National Health and Medical Research Council and Juvenile Diabetes Research Foundation International. It was also made possible through Victorian State Government Operational Infrastructure Support and Australian National Health and Medical Research Council Research Institute Infrastructure Support Scheme. L.C.H. was the recipient of a National Health and Medical Research Council Senior Principal Research Fellowship (1080887). P.A. received an Albert Renold Travel Fellowship from the European Foundation for the Study of Diabetes.