Absence of vitamin D receptor in mature osteoclasts results in altered osteoclastic activity and bone loss

Y Starczak; DC Reinke; KR Barratt; JW Ryan; PK Russell; MV Clarke; R St-Arnaud; HA Morris; RA Davey; GJ Atkins; PH Anderson

Journal article

Absence of vitamin D receptor in mature osteoclasts results in altered osteoclastic activity and bone loss

Y Starczak, DC Reinke, KR Barratt, JW Ryan, PK Russell, MV Clarke, R St-Arnaud, HA Morris, RA Davey, GJ Atkins, PH Anderson

Journal of Steroid Biochemistry and Molecular Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2018

DOI: 10.1016/j.jsbmb.2017.10.022

Abstract

Mature osteoclasts express the vitamin D receptor (VDR) and are able to synthesise and respond to 1,25(OH) 2 D 3 via CYP27B1 enzyme activity. Whether vitamin D signalling within osteoclasts is necessary for the regulation of osteoclastic bone resorption in an in vivo setting is unclear. To determine the requirement for the VDR- and CYP27B1-mediated activity in mature osteoclasts, conditional deletion mouse models were created whereby either Vdr or Cyp27b1 gene was inactivated by breeding either Vdr fl/fl or Cyp27b1 fl/fl mice with Cathepsin K-Cre transgenic mice (Cstk Cre ) to generate Ctsk Cre /Vdr −/− and Ctsk Cre /Cyp27b1 −/− mice respectively. To account for potential Ctsk Cre -meaited o..

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University of Melbourne Researchers

Michele Clarke Author

Rachel Davey Author

Grants

Awarded by University of Melbourne

Funding Acknowledgements

We would like to acknowledge the contributions of Michele Clarke and Patricia Russel (University of Melbourne) for the assistance in the creation of mice and Rebecca Sawyer (University of South Australia) for assistance in the histological analyses. This study was supported by funding from the National Health and Medical Research Council of Australia (NHMRC), GNT1029926. PHA was supported by and NHMRC RD Wright Fellowship and GJA is an NHMRC Senior Research Fellow.