Non-Neurotoxic Nanodiamond Probes for Intraneuronal Temperature Mapping

David A Simpson; Emma Morrisroe; Julia M McCoey; Alain H Lombard; Dulini C Mendis; Francois Treussart; Liam T Hall; Steven Petrou; Lloyd CL Hollenberg

Journal article

Non-Neurotoxic Nanodiamond Probes for Intraneuronal Temperature Mapping

David A Simpson, Emma Morrisroe, Julia M McCoey, Alain H Lombard, Dulini C Mendis, Francois Treussart, Liam T Hall, Steven Petrou, Lloyd CL Hollenberg

ACS NANO | AMER CHEMICAL SOC | Published : 2017

DOI: 10.1021/acsnano.7b04850

Abstract

Optical biomarkers have been used extensively for intracellular imaging with high spatial and temporal resolution. Extending the modality of these probes is a key driver in cell biology. In recent years, the nitrogen-vacancy (NV) center in nanodiamond has emerged as a promising candidate for bioimaging and biosensing with low cytotoxicity and stable photoluminescence. Here we study the electrophysiological effects of this quantum probe in primary cortical neurons. Multielectrode array recordings across five replicate studies showed no statistically significant difference in 25 network parameters when nanodiamonds are added at varying concentrations over various time periods, 12-36 h. The phy..

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University of Melbourne Researchers

Steven Petrou Author Florey Department of Neuroscience and Mental Health

Lloyd Hollenberg Author Physics

Liam Hall Author Chemistry

David Simpson Author Physics

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Grants

Awarded by Australian Research Council Centre of Excellence for Quantum Computation and Communication Technology

Awarded by Australian Research Council Laureate Fellowship Scheme

Awarded by NHMRC fellowship scheme

Funding Acknowledgements

The authors acknowledge Prof. Michel Simonneau for helpful discussions regarding applications of the technology, Dr. Philipp Reineck for performing the DLS and zeta potential measurements on the ND solutions, and Dr. Babak Nasr for the helium ion microscope images. This research was supported in part by the Australian Research Council Centre of Excellence for Quantum Computation and Communication Technology (project number CE110001027). This work was also supported by the University of Melbourne through the Centre for Neural Engineering and the Centre for Neuroscience. L.C.L.H. acknowledges the support of the Australian Research Council Laureate Fellowship Scheme (FL130100119). S.P. acknowledges the support from the NHMRC fellowship scheme (1005050). D.A.S. acknowledges support from the Melbourne Neuroscience Institute Fellowship Scheme.