Journal article
Identification of isolates of the plant pathogen Leptosphaeria maculans with resistance to the triazole fungicide fluquinconazole using a novel in planta assay
AP Van De Wouw, VL Elliott, S Chang, FJ López-Ruiz, SJ Marcroft, A Idnurm
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2017
Open access
Abstract
Leptosphaeria maculans is the major pathogen of canola (oilseed rape, Brassica napus) worldwide. In Australia, the use of azole fungicides has contributed to the 50-fold increase in canola production in the last 25 years. However, extensive application of fungicides sets the stage for the selection of fungal populations with resistance. A high-throughput in planta assay was developed to allow screening of thousands of isolates from multiple populations. Using this screen, isolates were identified with decreased sensitivity to the fungicide fluquinconazole when applied at field rates as a protective seed dressing: these isolates cause significantly larger lesions on cotyledons and true leaves..
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Awarded by Grains Research and Development Corporation
Funding Acknowledgements
All research was funded by the Grains Research and Development Corporation through UM00051 to Alexander Idnurm and CUR00023 to Francisco J Lopez-Ruiz. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank the Australian Grains Research and Development Corporation for funding this research and the agronomists, farmers and researchers that contributed stubble for screening in the large-scale survey.