Journal article
Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB
SN Willis, J Tellier, Y Liao, S Trezise, A Light, K O'Donnell, LA Garrett-Sinha, W Shi, DM Tarlinton, SL Nutt
Nature Communications | NATURE PUBLISHING GROUP | Published : 2017
Abstract
Humoral immunity requires B cells to respond to multiple stimuli, including antigen, membrane and soluble ligands, and microbial products. Ets family transcription factors regulate many aspects of haematopoiesis, although their functions in humoral immunity are difficult to decipher as a result of redundancy between the family members. Here we show that mice lacking both PU.1 and SpiB in mature B cells do not generate germinal centers and high-Affinity antibody after protein immunization. PU.1 and SpiB double-deficient B cells have a survival defect after engagement of CD40 or Toll-like receptors (TLR), despite paradoxically enhanced plasma cell differentiation. PU.1 and SpiB regulate the ex..
View full abstractGrants
Awarded by National Science Foundation
Funding Acknowledgements
We thank Patrick Leung and Nadia Iannarella for technical assistance and Meinrad Busslinger (IMP Vienna) for mice. This work was supported by a National Health and Medical Research Council (NHMRC) of Australia grants (1054925 to D.M.T. and S.L.N., 1058238 to S.L.N. and 1060675 to D.M.T.), Victorian State Government Operational Infrastructure Support, Australian Government NHMRC IRIIS, Lupus Research Alliance (to D.M.T.) and the Multiple Myeloma Research Foundation (MMRF, to S.L.N. and J.T.). S.N.W. was supported by a Walter and Eliza Hall Trust Centenary Fellowship, W.S. by a Commonwealth Serum Laboratories Centenary Fellowship.