Journal article
Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling
B Pal, Y Chen, F Vaillant, P Jamieson, L Gordon, AC Rios, S Wilcox, N Fu, KH Liu, FC Jackling, MJ Davis, GJ Lindeman, GK Smyth, JE Visvader
Nature Communications | NATURE PORTFOLIO | Published : 2017
Open access
Abstract
The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling of mouse mammary epithelial cells spanning four developmental stages in the post-natal gland. Notably, the epithelium undergoes a large-scale shift in gene expression from a relatively homogeneous basal-like program in pre-puberty to distinct lineage-restricted programs in puberty. Interrogation of single-cell transcriptomes reveals different levels of diversity within the luminal and basal compartments, and identifies an early progenitor subset marked by C..
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Awarded by Ian Potter Foundation
Funding Acknowledgements
We are grateful to L. Whitehead for assistance with confocal analysis, G. Wahl for sharing the single-cell qPCR method, B. Capaldo for imaging assistance, K. Hogg, and the Imaging, Animal and FACS facilities at WEHI. This work was supported by the Australian National Health and Medical Research Council (NHMRC) grants #1016701, #1054618, #1100807, #1113133; NHMRC IRIISS; the Victorian State Government through VCA funding of the Victorian Breast Cancer Research Consortium and Operational Infrastructure Support; the Australian Cancer Research Foundation and the Ian Potter Foundation. M.J.D., N.F. and A.C.R. were supported by a National Breast Cancer Foundation (NBCF) Fellowship; G.J.L. and G.K.S. by NHMRC Fellowships #1078730, #1058892; and J.E.V. by NHMRC Fellowships #1037230 and 1102742.