Journal article
Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal
SA Sinclair, C Larue, L Bonk, A Khan, H Castillo-Michel, RJ Stein, D Grolimund, D Begerow, U Neumann, MJ Haydon, U Krämer
Current Biology | CELL PRESS | Published : 2017
Abstract
Etiolated growth in darkness or the irreversible transition to photomorphogenesis in the light engages alternative developmental programs operating across all organs of a plant seedling. Dark-grown Arabidopsis de-etiolated by zinc (dez) mutants exhibit morphological, cellular, metabolic, and transcriptional characteristics of light-grown seedlings. We identify the causal mutation in TRICHOME BIREFRINGENCE encoding a putative acyl transferase. Pectin acetylation is decreased in dez, as previously found in the reduced wall acetylation2-3 mutant, shown here to phenocopy dez. Moreover, pectin of dez is excessively methylesterified. The addition of very short fragments of homogalacturonan, tri-ga..
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Awarded by Seventh Framework Programme
Funding Acknowledgements
We thank Petra Duchting (Ruhr University Bochum, Germany) for multielement analysis and the Paul Scherrer Institut (Villigen, Switzerland) and the European Synchrotron Radiation Source (ESRF, Grenoble, France) for beam-time at the microXAS beamline of the SLS and the ID21 beamlines, respectively. We thank Bradley Dotson and Chris Somerville (UC Berkeley) for sharing unpublished data, as well as Samantha Vernhettes and Herman Hofte (INRA Versailles, France), and Sascha Gille and Markus Pauly (UC Berkeley, CA), for sharing methods and for discussions. Funding was from the Deutsche Forschungsgemeinschaft (Kr1967/5-1 and 15-1; INST 213/803-1 FUGG), Ruhr University Bochum, and the European Union's Seventh Framework Program (FP7/2007-2013) under REA grant agreement nos. PIIF-GA-2008-219457 and PIEF-GA-2012-331680 (Marie Curie Actions) and CALIPSO (grant no. 312284).