Journal article

Eph-ephrin signaling modulated by polymerization and condensation of receptors

Samuel Ojosnegros, Francesco Cutrale, Daniel Rodriguez, Jason J Otterstrom, Chi Li Chiu, Veronica Hortiguela, Carolina Tarantino, Anna Seriola, Stephen Mieruszynski, Elena Martinez, Melike Lakadamyali, Angel Raya, Scott E Fraser

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2017

Abstract

Eph receptor signaling plays key roles in vertebrate tissue boundary formation, axonal pathfinding, and stem cell regeneration by steering cells to positions defined by its ligand ephrin. Some of the key events in Eph-ephrin signaling are understood: ephrin binding triggers the clustering of the Eph receptor, fostering transphosphorylation and signal transduction into the cell. However, a quantitative and mechanistic understanding of how the signal is processed by the recipient cell into precise and proportional responses is largely lacking. Studying Eph activation kinetics requires spatiotemporal data on the number and distribution of receptor oligomers, which is beyond the quantitative pow..

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Grants

Awarded by Marie Curie International Outgoing Fellowship within the EU Seventh Framework Programme


Awarded by Human Frontier Science Program Organization


Awarded by Ministerio de Educacion, Programa Nacional de Movilidad de Recursos Humanos del Plan Nacional de I-D+i


Awarded by NIH


Awarded by Generalitat de Catalunya


Awarded by Spanish Ministry of Economy and Competitiveness


Awarded by Instituto de Salud Carlos III (ISCIII)/FEDER


Awarded by European Union (GLAM)


Awarded by European Research Council


Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT


Awarded by NATIONAL INSTITUTE ON DRUG ABUSE


Funding Acknowledgements

The authors thank Giulia Ossato, William Dempsey, and Nicolas Plachta for useful discussions, and acknowledge the Nikon Center of Excellence at ICFO-The Institute of Photonic Sciences. S.O. was supported by Marie Curie International Outgoing Fellowship 276282 within the EU Seventh Framework Programme FP7/2007-2013 and Postdoctoral Fellowships LT000109/2011 from the Human Frontier Science Program Organization and EX2009-1136 from the Ministerio de Educacion, Programa Nacional de Movilidad de Recursos Humanos del Plan Nacional de I-D+i 2008-2011. F.C. was supported by grants from the Moore Foundation and NIH (R01 HD075605 and R01 OD019037). J.J.O. acknowledges financial support from ICFONEST+, funded by the Marie Curie COFUND (FP7-PEOPLE-2010-COFUND) action of the European Commission and by the MINECO Severo Ochoa action at ICFO-The Institute of Photonic Sciences. Additional funding was provided by the Generalitat de Catalunya (2014-SGR-1442 and 2014-SGR-1460); the Spanish Ministry of Economy and Competitiveness (SAF2015-69706-R, MINAHE5, TEC2014-51940-C2-2-R, SEV-2015-0522); Instituto de Salud Carlos III (ISCIII)/FEDER (RD16/0011/0024); the European Union (GLAM project GA-634928; System's Microscopy Network of Excellence consortium FP-7-HEALTH.2010.2.1.2.2); the European Research Council (337191-MOTORS and 647863-COMIET); the Fundacio Privada Cellex; and CERCA Programme/Generalitat de Catalunya.