Journal article

Experimental study of delivery of humidified-warm carbon dioxide during open abdominal surgery

S Carpinteri, S Sampurno, J Malaterre, R Millen, M Dean, J Kong, T Chittleborough, A Heriot, AC Lynch, RG Ramsay



BACKGROUND: The aim of this study was to monitor the effect of humidified-warm carbon dioxide (HWCO2 ) delivered into the open abdomen of mice, simulating laparotomy. METHODS: Mice were anaesthetized, ventilated and subjected to an abdominal incision followed by wound retraction. In the experimental group, a diffuser device was used to deliver HWCO2 ; the control group was exposed to passive air flow. In each group of mice, surgical damage was produced on one side of the peritoneal wall. Vital signs and core temperature were monitored throughout the 1-h procedure. The peritoneum was closed and mice were allowed to recover for 24 h or 10 days. Tumour cells were delivered into half of the mice..

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Funding Acknowledgements

S.C. and S.S. contributed equally to this study. This work was supported by the National Health and Medical Research Council, Australia, Fisher and Paykel Healthcare, the Peter MacCallum Cancer Centre Foundation and the Epworth Research Foundation. Special thanks to J. Hiller, who was instrumental in optimizing the animal anaesthesia conditions required for the open surgery procedures employed in this study.