Journal article
Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors
EYN Lam, JYM Chau, ML Kalev-Zylinska, TM Fountaine, RS Mead, CJ Hall, PS Crosier, KE Crosier, MV Flores
Blood | AMER SOC HEMATOLOGY | Published : 2009
Abstract
The transcription factor Runx1 is essential for the development of definitive hematopoietic stem cells (HSCs) during vertebrate embryogenesis and is transcribed from 2 promoters, P1 and P2, generating 2 major Runx1 isoforms. We have created 2 stable runx1 promoter zebrafish-transgenic lines that provide insight into the rolesof theP1 and P2isoforms during the establishment of definitive hematopoiesis. The Tg(runx1P1:EGFP) line displays fluorescence in the posterior blood island, where definitive erythromyeloid progenitors develop. The Tg(runx1P2:EGFP) line marks definitive HSCs in the aortagonad-mesonephros, with enhanced green fluorescent protein-labeled cells later populating the pronephro..
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Funding Acknowledgements
[ "The authors thank L. Khan for technical assistance, A. Mahagaonkar for managing the zebrafish facility, S. Edgar for FACS analysis support, and the University of Auckland Biomedical Imaging Research Unit for their expert support with confocal microscopy. The Tol2/transposase expression vectors and the Tg(gata1:DsRed) transgenics were gifts from K. Kawakami and D. Traver, respectively.", "This work was supported by grants from the Auckland Medical Research Foundation and the New Economy Research Fund, Foundation for Research, Science and Technology. E.Y.N.L. is a Bright Futures Top Achiever Doctoral Scholar." ]