Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake
Philip J Ryan, Silvano I Ross, Carlos A Campos, Victor A Derkach, Richard D Palmiter
NATURE NEUROSCIENCE | NATURE PUBLISHING GROUP | Published : 2017
Brain regions that regulate fluid satiation are not well characterized, yet are essential for understanding fluid homeostasis. We found that oxytocin-receptor-expressing neurons in the parabrachial nucleus of mice (OxtrPBN neurons) are key regulators of fluid satiation. Chemogenetic activation of OxtrPBN neurons robustly suppressed noncaloric fluid intake, but did not decrease food intake after fasting or salt intake following salt depletion; inactivation increased saline intake after dehydration and hypertonic saline injection. Under physiological conditions, OxtrPBN neurons were activated by fluid satiation and hypertonic saline injection. OxtrPBN neurons were directly innervated by oxytoc..View full abstract
Awarded by US National Institutes of Health
Awarded by NATIONAL INSTITUTE ON DRUG ABUSE
We thank B. Roth and K. Deisseroth for AAV plasmid constructs. M. Chiang assisted with animal husbandry. S. Tsang and K. Kafer helped to generate the Oxtr<SUP>Cre</SUP> mice. We thank J. Chen for help with surgery for Calca<SUP>Cre/+</SUP> experiments and C. Roman for help with surgery for Cck<SUP>Cre/+</SUP> experiments. M. McKinley, B. Jarvie, C. Roman and members of the Palmiter lab provided helpful discussion and feedback. P.J.R. was supported by an Australian American Fellowship and a National Health and Medical Council of Australia CJ Martin Fellowship. C.A.C. was supported by a fellowship from Hope Funds for Cancer Research. R.D.P. was supported by a US National Institutes of Health grant (R01-DA24908). Inscopix provided the calcium-imaging equipment and supplies via their DECODE grant program.