Transforming growth factor-beta 1-mediated renal fibrosis is dependent on the regulation of transforming growth factor receptor 1 expression by let-7b
Bo Wang, Jay C Jha, Shinji Hagiwara, Aaron D McClelland, Karin Jandeleit-Dahm, Merlin C Thomas, Mark E Cooper, Phillip Kantharidis
KIDNEY INTERNATIONAL | ELSEVIER SCIENCE INC | Published : 2014
Renal fibrosis results from excessive accumulation of extracellular matrix mainly driven by transforming growth factor-β1 (TGF-β1). Certain microRNAs have been implicated in this disease, and here we examine the role of let-7b. Rat proximal tubular epithelial cells (NRK52E) were treated with TGF-β1 for 3 days to assess the expression of markers of fibrosis and let-7b. These factors were also assessed in two mouse models representing early and more advanced diabetic nephropathy and in the non-diabetic adenine-induced renal fibrosis model. TGF-β1 downregulated the expression of let-7b and induced fibrogenesis in NRK52E cells. Ectopic expression of let-7b repressed TGF-β1 receptor 1 (TGFBR1) ex..View full abstract
Awarded by National Health and Medical Research Council of Australia
Awarded by Heart Foundation
This study was supported by a grant from the National Health and Medical Research Council of Australia (NHMRC526663), the Heart Foundation (G11M 6134), the Diabetes Australia Research Trust, a Skip Martin Award, and an Australia Fellowship for MEC. PK is supported by the Vera Dalgleish Phillips Fellowship. This study was supported by a Skip Martin Award from the Diabetes Australia Research Trust, and an Australia Fellowship from the National Health and Medical Research Council.