Journal article

Dedifferentiation of Immortalized Human Podocytes in Response to Transforming Growth Factor-beta A Model for Diabetic Podocytopathy

Michal Herman-Edelstein, Merlin C Thomas, Vicki Thallas-Bonke, Moin Saleem, Mark E Cooper, Phillip Kantharidis

DIABETES | AMER DIABETES ASSOC | Published : 2011

Abstract

OBJECTIVE: Diabetic nephropathy is associated with dedifferentiation of podocytes, losing the specialized features required for efficient glomerular function and acquiring a number of profibrotic, proinflammatory, and proliferative features. These result from tight junction and cytoskeletal rearrangement, augmented proliferation, and apoptosis. RESEARCH DESIGN AND METHODS: Experiments were performed in conditionally immortalized human podocytes developed by transfection with the temperature-sensitive SV40-T gene. Cells were then cultured in the presence of transforming growth factor (TGF)-β1 or angiotensin II in the presence or absence of a selective inhibitor of the TGF-β type I receptor ki..

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University of Melbourne Researchers