Dedifferentiation of Immortalized Human Podocytes in Response to Transforming Growth Factor-beta A Model for Diabetic Podocytopathy
Michal Herman-Edelstein, Merlin C Thomas, Vicki Thallas-Bonke, Moin Saleem, Mark E Cooper, Phillip Kantharidis
DIABETES | AMER DIABETES ASSOC | Published : 2011
OBJECTIVE: Diabetic nephropathy is associated with dedifferentiation of podocytes, losing the specialized features required for efficient glomerular function and acquiring a number of profibrotic, proinflammatory, and proliferative features. These result from tight junction and cytoskeletal rearrangement, augmented proliferation, and apoptosis. RESEARCH DESIGN AND METHODS: Experiments were performed in conditionally immortalized human podocytes developed by transfection with the temperature-sensitive SV40-T gene. Cells were then cultured in the presence of transforming growth factor (TGF)-β1 or angiotensin II in the presence or absence of a selective inhibitor of the TGF-β type I receptor ki..View full abstract
This work received research funding from the Kidney Health Australia (Bootle bequest), Juvenile Diabetes Research Foundation, and National Health and Medical Research Council of Australia.